Close
Shop All:Innovating Science
Innovating Science AP Biology 9 Biotechnology Restriction Enzyme Analysis
Item #:
2134306
$187.39
$140.54
Ships Directly from Manufacturer Typically Within 2-4 Weeks - Lead Times Vary
School Specialty Shipping Policy
Items Shipped Within the Contiguous 48 United States
Free Shipping Eligible Items
- Free shipping and handling on eligible supply orders of $69 or more. Free shipping calculation is based on the subtotals of eligible items, after any additional discounts are applied.
- For subtotals less than $69, the shipping and handling charge is $11.95.
Free Shipping Ineligible Items
- Shipping and handling charges are 20% of the subtotal of the items, after any discounts are applied, with a $11.95 minimum charge.
Free Shipping Ineligible Items
- Shipping and handling charges are 20% of the subtotal of the items, after any discounts are applied, with a $99 minimum charge. If the subtotal is greater than $1,000, please e-mail freight_quote@schoolspecialty.com for a freight quote.
*Note: Individually negotiated shipping policies will override the standard shipping policy. See Full Details
Items Shipped to Hawaii, Alaska and International
See our Hawaii & Alaska Shipping Policy and International Shipping Policy for details.
In this lab students will understand the action and specificity of restriction enzymes, while learning the components involved in the process of DNA electrophoresis and the role of each component as it applies to the entire process of electrophoresis.
About This Item
Description
Students will perform the electrophoresis process on DNA samples treated with different restriction enzymes and construct a standard curve using a known DNA sample while determining the approximate size of the DNA fragments in unknown samples. Meets AP Science Practices 3 and 6, and Big Idea 3. Materials provided for eight lab groups.Features
Materials Needed but not Supplied:
- Agarose electrophoresis chamber Microwave or hot water bath
- Electrophoresis power supply Distilled or deionized water
- Micropipettes w/micropipette tips, 10ul
- Gel staining trays or similar (large enough container to accommodate flat agarose gels)
Includes:
- 200mL Prepared Agarose, 0.8%
- 500mL TBE buffer, 5X
- 60mL DNA Stain, 20X
- DNA Samples, 100ul each
- DNA Marker (Lambda DNA HindIII digest)
- Sample #1 (Lambda DNA)
- Sample #2 (Lambda DNA BstEII digest)
- Sample #3 (Lambda DNA EcoRI digest)
- Sample #4 (Lambda DNA BstEII digest)
Innovating Science AP Biology 9 Biotechnology Restriction Enzyme Analysis