• Home
  • Innovating Science Electrophoresis and DNA Fragment Length Determination

Innovating Science Electrophoresis and DNA Fragment Length Determination

Image for Innovating Science Electrophoresis and DNA Fragment Length Determination from School Specialty
Item #: 2134316
$156.19
$117.14
Ships Directly from Manufacturer Typically Within 2-4 Weeks - Lead Times Vary
Free Shipping Eligible: Details

School Specialty Shipping Policy

Items Shipped Within the Contiguous 48 United States

Free Shipping Eligible Items

  • Free shipping and handling on eligible supply orders of $69 or more. Free shipping calculation is based on the subtotals of eligible items, after any additional discounts are applied.
  • For subtotals less than $69, the shipping and handling charge is $11.95.

Free Shipping Ineligible Items

  • Shipping and handling charges are 20% of the subtotal of the items, after any discounts are applied, with a $11.95 minimum charge.

Free Shipping Ineligible Items

  • Shipping and handling charges are 20% of the subtotal of the items, after any discounts are applied, with a $99 minimum charge. If the subtotal is greater than $1,000, please e-mail freight_quote@schoolspecialty.com for a freight quote.

*Note: Individually negotiated shipping policies will override the standard shipping policy. See Full Details

Items Shipped to Hawaii, Alaska and International

See our Hawaii & Alaska Shipping Policy and International Shipping Policy for details.

Students will learn about the process of DNA electrophoresis .

Select options to add to cart.
Select options to add to list.
About This Item

Description

Restriction enzymes are enzymes that are capable to cleaving, or cutting, DNA molecules in very specific spots based on the DNA molecule's sequence of bases. Their discovery and isolation in the latter half of the 20th century provided scientists with an invaluable tool and rapidly expanded in the field of molecular biology. Agarose electrophoresis is a technique for separating the pieces of DNA that result from the use of restriction enzymes. In this activity students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes. One sample has been cut with the enzyme HindIII, one has been cut with the enzyme EcoRI, and one has been cut with both enzymes. The resulting pattern of DNA bands on the agarose gel will show that each enzyme affects the DNA differently. Students will not only learn about the process of DNA electrophoresis but learn the techniques associated with the process, such as setting up an agarose gel, loading DNA samples in the agarose gel, and staining the gel in order to visualize the DNA bands. They will also learn how to determine the sizes of unknown DNA fragments after examining their results.

Features

Materials Needed but not Supplied:

  • Agarose electrophoresis chamber Microwave or hot water bath
  • Electrophoresis power supply Distilled or deionized water
  • Micropipettes w/micropipette tips, 10uL
Includes:
  • 200mL Prepared Agarose, 0.8% Solution
  • 500mL Tris Borate ETA Buffer, 5X
  • 60mL DNA Stain, 20X
  • 100uL DNA/EcoRI Digest with Loading Dye
  • 100 uL Lambda DNA/HindIII Digest with Loading Dye
  • 100 uL Lambda DNA/EcoRI,HindIII Digest with Loading Dye
Shipping Details
Shipping Type: parcel
Free Shipping: true
Specifications
Maximum Age: 18+
Maximum Grade Level: HS+
Allergens: Contains No Allergens
Certifications: ISO Certified
Grade Level: Grades 6-12
Safety: Caution Icon. No Choking Hazard
SUOM Description: Each
Age Range: 12+
Brand Name: Innovating Science
Description: DNA Fragment Length Kit
Innovating Science Electrophoresis and DNA Fragment Length Determination