Innovating Science Electrophoresis and DNA Fragment Length Determination

Innovation Science Electrophoresis and DNA Fragment Length Determination 2134316

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Innovating Science Electrophoresis and DNA Fragment Length Determination | School Specialty

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<div class="long-description"><h2>Description</h2>Restriction enzymes are enzymes that are capable to cleaving, or cutting, DNA molecules in very specific spots based on the DNA molecule's sequence of bases. Their discovery and isolation in the latter half of the 20th century provided scientists with an invaluable tool and rapidly expanded in the field of molecular biology. Agarose electrophoresis is a technique for separating the pieces of DNA that result from the use of restriction enzymes. In this activity students will perform agarose electrophoresis on three DNA samples that have already been treated with restriction enzymes. One sample has been cut with the enzyme HindIII, one has been cut with the enzyme EcoRI, and one has been cut with both enzymes. The resulting pattern of DNA bands on the agarose gel will show that each enzyme affects the DNA differently. Students will not only learn about the process of DNA electrophoresis but learn the techniques associated with the process, such as setting up an agarose gel, loading DNA samples in the agarose gel, and staining the gel in order to visualize the DNA bands. They will also learn how to determine the sizes of unknown DNA fragments after examining their results.</div><div class="feature-description"><h2>Features</h2><p>Materials Needed but not Supplied:</p> <ul> <li>Agarose electrophoresis chamber Microwave or hot water bath</li> <li>Electrophoresis power supply Distilled or deionized water</li> <li>Micropipettes w/micropipette tips, 10uL</li> </ul></div><div class="in-box-description"><b>Includes:</b> <ul> <li>200mL Prepared Agarose, 0.8% Solution</li> <li>500mL Tris Borate ETA Buffer, 5X</li> <li>60mL DNA Stain, 20X</li> <li>100uL DNA/EcoRI Digest with Loading Dye</li> <li>100 uL Lambda DNA/HindIII Digest with Loading Dye</li> <li>100 uL Lambda DNA/EcoRI,HindIII Digest with Loading Dye</li> </ul></div>

Students will learn about the process of DNA electrophoresis .

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Innovating Science Electrophoresis and DNA Fragment Length Determination

$138.37

Students will learn about the process of DNA electrophoresis .

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